Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield
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Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield
BACKGROUND The production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically eukaryotic protein production experiments have relied on varying expression construct cassettes such as promoters and tags, or culture process parameters such as pH, temperature and aeration to enhance yields. ...
متن کاملOn the Ribosomal Density that Maximizes Protein Translation Rate
During mRNA translation, several ribosomes attach to the same mRNA molecule simultaneously translating it into a protein. This pipelining increases the protein translation rate. A natural and important question is what ribosomal density maximizes the protein translation rate. Using mathematical models of ribosome flow along both a linear and a circular mRNA molecules we prove that typically the...
متن کاملCorrection: On the Ribosomal Density that Maximizes Protein Translation Rate
[This corrects the article DOI: 10.1371/journal.pone.0166481.].
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2009
ISSN: 1475-2859
DOI: 10.1186/1475-2859-8-10